OUTDOOR ALGAE

· 1.Sea water is filtered throw a 30 micron
· 2. Sea water is chlorinated by 75 ppm chlorine 5% for
· 24 hours
·2. Dechlorination with addition 75 ppm sodium
· thiosulphate
· 3.Nutrients can then be added according to the table


NUTRUIENTS FOR OUTDOORALGAE CULTURES

Compound                                                Amount

Amonium sulphate                                                        100 grams

Monopotassium phosphate                                            30 grams

Urea                                                                                   5 grams

Fe-EDTA                                                                        15 grams

Trace mineral mix                                                              4grams

Inoculation from the algae room as follow


150 L - Cylinder
1000 L - Tank
10000 L - Tank
Initial stocking density should be 3 to 5 million cells per ml .Harvest when density 20 to 25 million cells per ml
· Culture tank should be scabbed clean and rinsed between each use. DO NOT need to be chlorinated .Chlorination is not recommended because any residual chlorine can have detrimental effect on the rotifers and fish larvae


SOURCES OF CONTAMINATION
1. Air supply system
2 Water supply system
3. Culture tank
4. Dirty hands
5. Glassware
6. Mouth
7. Started culture
8. Air stone
9. Rain
10.Water source

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CONTAMINATION
Several types of non-algae contamination occur in micro algae culture
1. CILIATE:
* Paramecium
* Colpoda
* Euplotes

2-BCTERIA

3-NEMATODES

4- FUNGI

5-ROTIFERS