Thursday, June 28, 2012
The Marine Hachery: The hatching of Mugil Cephalus
The Marine Hachery: The hatching of Mugil Cephalus: The hatching of Mugil Cephalus The first injection : - HCG by a rate of 10,000 IU and attend as follows : Add 1 ml of sali...
Tuesday, June 26, 2012
OUTDOOR ALGAE
· 1.Sea water is filtered throw a 30 micron
· 2. Sea water is chlorinated by 75 ppm chlorine 5% for
· 24 hours
·2. Dechlorination with addition 75 ppm sodium
· thiosulphate
· 3.Nutrients can then be added according to the table
NUTRUIENTS FOR OUTDOORALGAE CULTURES
Compound
Amount
Amonium
sulphate
100 grams
Monopotassium
phosphate
30 grams
Urea
5 grams
Fe-EDTA 15
grams
Trace mineral
mix
4grams
Inoculation from the
algae room as follow
150 L - Cylinder
1000 L - Tank
10000 L - Tank
Initial stocking density should be 3 to 5 million cells per ml .Harvest when density 20 to 25 million cells per ml
· Culture tank should be scabbed clean and rinsed between each use. DO NOT need to be chlorinated .Chlorination is not recommended because any residual chlorine can have detrimental effect on the rotifers and fish larvae
SOURCES OF CONTAMINATION
1. Air supply system
2 Water supply system
3. Culture tank
4. Dirty hands
5. Glassware
6. Mouth
7. Started culture
8. Air stone
9. Rain
10.Water source
****************
CONTAMINATION
Several types of non-algae contamination occur in micro algae culture
1. CILIATE:
* Paramecium
* Colpoda
* Euplotes
2-BCTERIA
3-NEMATODES
4- FUNGI
5-ROTIFERS
150 L - Cylinder
1000 L - Tank
10000 L - Tank
Initial stocking density should be 3 to 5 million cells per ml .Harvest when density 20 to 25 million cells per ml
· Culture tank should be scabbed clean and rinsed between each use. DO NOT need to be chlorinated .Chlorination is not recommended because any residual chlorine can have detrimental effect on the rotifers and fish larvae
SOURCES OF CONTAMINATION
1. Air supply system
2 Water supply system
3. Culture tank
4. Dirty hands
5. Glassware
6. Mouth
7. Started culture
8. Air stone
9. Rain
10.Water source
****************
CONTAMINATION
Several types of non-algae contamination occur in micro algae culture
1. CILIATE:
* Paramecium
* Colpoda
* Euplotes
2-BCTERIA
3-NEMATODES
4- FUNGI
5-ROTIFERS
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