The Marine Hachery: The hatching of Mugil Cephalus

The Marine Hachery: The hatching of Mugil Cephalus: The hatching of Mugil Cephalus     The first injection : - HCG by a rate of 10,000 IU and attend as follows : Add 1 ml of sali...

                                 

OUTDOOR ALGAE

· 1.Sea water is filtered throw a 30 micron
· 2. Sea water is chlorinated by 75 ppm chlorine 5% for
· 24 hours
·2. Dechlorination with addition 75 ppm sodium
· thiosulphate
· 3.Nutrients can then be added according to the table


NUTRUIENTS FOR OUTDOORALGAE CULTURES

Compound                                                Amount

Amonium sulphate                                                        100 grams

Monopotassium phosphate                                            30 grams

Urea                                                                                   5 grams

Fe-EDTA                                                                        15 grams

Trace mineral mix                                                              4grams

Inoculation from the algae room as follow


150 L - Cylinder
1000 L - Tank
10000 L - Tank
Initial stocking density should be 3 to 5 million cells per ml .Harvest when density 20 to 25 million cells per ml
· Culture tank should be scabbed clean and rinsed between each use. DO NOT need to be chlorinated .Chlorination is not recommended because any residual chlorine can have detrimental effect on the rotifers and fish larvae


SOURCES OF CONTAMINATION
1. Air supply system
2 Water supply system
3. Culture tank
4. Dirty hands
5. Glassware
6. Mouth
7. Started culture
8. Air stone
9. Rain
10.Water source

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CONTAMINATION
Several types of non-algae contamination occur in micro algae culture
1. CILIATE:
* Paramecium
* Colpoda
* Euplotes

2-BCTERIA

3-NEMATODES

4- FUNGI

5-ROTIFERS

The hatching of Mugil Cephalus

The hatching of Mugil Cephalus   

The first injection: -
HCG by a rate of 10,000 IU and attend as follows:
Add 1 ml of saline solution 0.9 mg to an explosive hormone and is shaken and the withdrawal of 1 ml and injected by the female in fish muscle in the dorsal fin down the right side.
The purpose of this injection is that it increases the secretion of the hormone Gonadotropin Harmone (GTH)
And alert to dismantle the ovarian eggs
Second injection: -
24 hours after the first injection are injected into the mother at 200 micrograms of LHRH-a and equipped as follows:
Add 2 ml of saline solution 0.9 mg to 1 mg bottle hormone (1000 micrograms)
The amount of hormone necessary for fish 1 kg as follows:
Required dose = weight of fish in kg × 200/1000 × 2/1 = 0.4 mm / kg fish
The purpose of this hormone has an effect 50 times the capacity of the hormone
Gonadotropin A eleasing Hormone (GnRH) ovulation, it happens at a temperature of 23 to 25 after 16 to 20 hours
Placed 2 males and one female n the spawning tank